Letter | Published:

Induction of Thymine Dimers in Synchronized Populations of Chinese Hamster Cells

Nature volume 212, pages 298300 (15 October 1966) | Download Citation

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Abstract

RECENT evidence indicates that the response of Chinese hamster cells in vitro to ultra-violet radiation changes during the difference phases of the cell cycle1,2,4. Humphrey et al.1 have shown that cells synthesizing deoxyribo-nucleic acid (DNA) at the time of irradiation using ultraviolet rays (S phase cells) sustain more chromosome damage than cells which were in the period before DNA synthesis (G1 or cells in the period after DNA synthesis (G2). Sinclair and Morton have demonstrated that the response to ultra-violet radiation in synchronous populations of cells as measured by cell death also fluctuates with the age of the irradiated cells; the fraction of surviving cells falls as the cells move from G1 into S phase, and rises as they move from S into G2 phase2. In an effort to explain these changes in radiation response during the cell cycle, the production of an ultra-violet photoproduct of DNA, the thymine dimer3 has been measured in synchronized population of Chinese hamster cells4.

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Author information

Affiliations

  1. Graduate School of Biomedical Sciences, University of Texas.

    • D. L. STEWARD
  2. M.D. Anderson Hospital and Tumor Institute, Department of Physics, University of Texas.

    • R. M. HUMPHREY

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https://doi.org/10.1038/212298b0

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