Letter | Published:

Layer Technique in Gel Filtration for estimating Differences in the Degree of Dissociation of Closely Related Proteins

Nature volume 212, pages 296297 (15 October 1966) | Download Citation

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Abstract

WHEN the interaction or the association of large molecules is to be studied by gel filtration1, it is recommended2–5 that enough solution should be passed into the gel column for a plateau region to appear on the elution record. The leading and trailing boundaries do not then interfere with each other, and the effective elution position of a constituent can be found by constructing an equivalent sharp boundary using Longsworth's6 procedure for homogeneous solutions. In rinding this position, to obtain what may be called the constituent elution volume i, all the forms of the constituent, combined and free, are included.

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Affiliations

  1. Department of Chemistry, University of Birmingham.

    • G. A. GILBERT

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DOI

https://doi.org/10.1038/212296a0

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