Letter | Published:

Molecular Size Studies on 6-Phosphogluconate Dehydrogenase

Nature volume 212, pages 197198 (08 October 1966) | Download Citation

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Abstract

GENETICALLY determined electrophoretic variants of 6-phosphogluconate dehydrogenase (6-PGD) have been found in man and rat1, pigeon2, deer mouse3, and the fruit fly4. In all five species the heterozygous phenotype contains the two parental enzyme forms plus a third 6-PGD of intermediate electrophoretic mobility on starch gel. In experiments with partially purified 6-PGD of Drosophila melanogaster we have recovered the intermediate PGD IAB after in vitro treatment of the two parental types, PGD A and PGD B, with propanedithiol5. Although this is good evidence that the enzyme is composed of two or more sub-units, the remote possibility that the two parental enzymes polymerize to form the intermediate PGD IAB was not excluded. We now report further experiments bearing on the molecular structure and size of the enzyme.

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References

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    , Nature, 210, 487 (1966).

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    , and (unpublished results).

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    , J. Hered. (in the press).

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    , Biochem., 3, 723 (1964).

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Author notes

    • HAIG H. KAZAZIAN JUN.

    Present address: Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland.

Affiliations

  1. Department of Pediatrics, Johns Hopkins Hospital.

    • HAIG H. KAZAZIAN JUN.

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https://doi.org/10.1038/212197a0

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