Letter | Published:

Effect of Actinomycin D and Halogenated Deoxyuridines on the Replication of Bittner Virus in vitro

Naturevolume 212pages100101 (1966) | Download Citation



THE mouse mammary tumour virus (MTV)—or Bittner virus—is an RNA virus containing lipid1 which seems to multiply in the cytoplasm of infected cells and to be released by a budding process of the plasma membrane. Virus is released continuously without causing death of the cells. It appears that in its interaction with the cell, the virus becomes part of a steady-state complex of the type defined by Dulbecco2. It was of interest to determine whether viral replication, under the conditions in the steady-state interaction, required the participation of functional DNA—such as appears to occur with the Rous sarcoma virus3,4, a moderate, lipid-containing RNA tumour virus—or whether, as with most RNA viruses, replication was not dependent on DNA.

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  1. 1

    Lyons, M. J., and Moore, D. H., J. Nat. Cancer Inst., 35, 549 (1965).

  2. 2

    Dulbecco, R., in Viral and Rickettsial Infection of Man, fourth ed., edit. by Horsfall, F., and Tamm, I., 267 (Lippincott Co., Philadelphia and Montreal, 1965).

  3. 3

    Temin, H. M., Virology, 20, 577 (1963).

  4. 4

    Bader, J. P., Virology, 22, 462 (1964).

  5. 5

    Reich, E., Franklin, R. M., Shatkin, A. J., and Tatum, E. L., Science, 134, 556 (1961).

  6. 6

    Goldberg, I. H., and Rabinowitz, M., Science, 136, 315 (1962).

  7. 7

    Cohen, S. S., Flaks, J. G., Barner, H. D., Leob, M. R., and Lichtenstein, J., Proc. U.S. Nat. Acad. Sci., 44, 1004 (1958).

  8. 8

    Hsu, T. C., and Somers, C. E., Proc. U.S. Nat. Acad. Sci., 47, 396 (1961).

  9. 9

    Dmochowski, L., Grey, C. E., Langford, P. L., Migliore, P. L., Sykes, J. A., Williams, W. C., and Young, E. L., in Carcinogenesis: A Broad Critique (Williams and Wilkins Co., Baltimore, in the press).

  10. 10

    Lasfargues, E. Y., Murray, M. R., and Moore, D. H., J. Nat. Cancer Inst., 34, 141 (1965).

  11. 11

    Ui, H., and Mueller, G. L., Proc. U.S. Nat. Acad. Sci., 50, 256 (1963).

  12. 12

    White, D. O., and Cheyne, I. M., Nature, 208, 813 (1965).

  13. 13

    Young, B. G., and Engels, B., Fed. Proc., 25, 477 (1966).

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    Present address: Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia


  1. The Rockefeller University, New York

    •  & PAUL E. CAME
  2. College of Physicians and Surgeons, Columbia University, New York

    •  & PAUL E. CAME


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