Letter | Published:

Rapid Quantitative Determination of Very Small Amounts of Proteins

Nature volume 212, pages 7576 (01 October 1966) | Download Citation

Subjects

Abstract

IN the course of chromatography of proteins on UFS nitrocellulose membrane filters1, it was observed that the areas occupied by a relatively homogenous film of the firmly adsorbed serum proteins were approximately proportional to the concentration of the given sample. The best results were achieved when slightly acid buffers were used. The protein spots, however, had oblong and irregular shapes after one-dimensional chromatography, and an accurate quantitative determination of the areas was rather difficult and inconvenient, especially as a routine procedure.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , J. Chromatog. (in the press).

  2. 2.

    , Biochim. Biophys. Acta, 117, 475 (1966).

  3. 3.

    , Aerztl. Lab., 10, 233 (1964).

  4. 4.

    , Základy Chemického Vyšetrovǎní v Lékarství, sixth ed., 298, 333, 354–360 (SZN, Praha, 1964).

  5. 5.

    , , , and , J. Lab. Clin. Med., 66, 334 (1965).

Download references

Author information

Affiliations

  1. Institute of Haematology and Blood Transfusion, Prague.

    • T. I. PŘISTOUPIL

Authors

  1. Search for T. I. PŘISTOUPIL in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/212075a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.