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Encephalitogenic Peptides from Spinal Cord

Nature volume 209, pages 1354–1355 (1966)Cite this article

Abstract

IN a recent symposium on experimental allergic encephalomyelitis there was considerable discussion on the size of encephalitogenic factors from brain and spinal cord1–4. We have fractionated extracts from bovine spinal cord by gel-filtration with phenol–acetic acid–water. This solvent system was introduced by Synge and colleagues5–7 for the fractionation of peptides on ‘Sephadex G-75’. It is a strongly dissociating solvent which prevents adsorption of peptides on ‘Sephadex’, and gel-filtration in this solvent can be used to estimate the molecular size of peptides as there is an approximately linear relationship between the relative elution volume and the log of molecular weight8,9. This communication describes a typical fractionation of basic polypeptides which were found to produce experimental allergic encephalomyelitis in guinea-pigs.

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References

  1. Kies, M. W., Thompson, E. B., and Alvord, E. C., Ann. N.Y. Acad. Sci., 122, 148 (1965).

    Article  ADS  CAS  Google Scholar 

  2. Kies, M. W., Ann. N.Y. Acad. Sci., 122, 161 (1965).

    Article  ADS  CAS  Google Scholar 

  3. Caspary, E. A., and Field, E. J., Ann. N.Y. Acad. Sci., 122, 182 (1965).

    Article  ADS  CAS  Google Scholar 

  4. Nakao, A., and Roboz-Einstein, E., Ann. N.Y. Acad. Sci., 122, 171 (1965).

    Article  ADS  CAS  Google Scholar 

  5. Synge, R. L. M., and Youngson, M. A., Biochem. J., 78, 31P (1961).

    Article  Google Scholar 

  6. Bagdasarian, M., Matheson, N. A., Synge, R. L. M., and Youngson, M. A., Biochem. J., 91, 91 (1964).

    Article  CAS  Google Scholar 

  7. Synge, R. L. M., Metabolism, 13, 5 (1964).

    Article  Google Scholar 

  8. Carnegie, P. R., Biochem. J., 95, 9P (1965).

    CAS  Google Scholar 

  9. Carnegie, P. R., Nature, 206, 1128 (1965).

    Article  ADS  CAS  Google Scholar 

  10. Craig, L. C., and King, T. P., Methods Biochem. Analysis, 10, 175 (1962).

    CAS  Google Scholar 

  11. Schonne, E., Protides of the Biological Fluids, 11, 368 (1964).

    CAS  Google Scholar 

  12. Lumsden, C. E., Robertson, D. M., and Blight, R., Z. Immunitäts. Allergieforsch., 126, 168 (1964).

    CAS  PubMed  Google Scholar 

  13. Margoliash, E., and Smith, E. L., J. Biol. Chem., 237, 2151 (1962).

    CAS  PubMed  Google Scholar 

  14. Kies, M. W., Ann. N.Y. Acad. Sci., 122, 194 (1965).

    Google Scholar 

  15. Hnilica, L. S., Experientia, 21, 124 (1965).

    Article  CAS  Google Scholar 

  16. Laatsch, R. H., Kies, M. W., Gordon, S., and Alvord, E. C., J. Exp. Med., 115, 777 (1962).

    Article  CAS  Google Scholar 

  17. Thompson, E. B., and Kies, M. W., Ann. N.Y. Acad. Sci., 122, 129 (1965).

    Article  ADS  CAS  Google Scholar 

  18. Soto, E. F., Neurology, 14, 938 (1964).

    Article  CAS  Google Scholar 

  19. Rauch, H. C., and Raffel, S., J. Immunol., 92, 452 (1964).

    CAS  PubMed  Google Scholar 

  20. Lumsden, C. E., Robertson, D. M., and Blight, R., J. Neurochem., 13, 127 (1966).

    Article  CAS  Google Scholar 

  21. Hottle, G. A., Nedzel, G. A., Wright, J. T., and Bell, J. F., Proc. Soc. Exp. Biol. and Med., 72, 289 (1949).

    Article  CAS  Google Scholar 

  22. Kibler, R. F., Fox, R. H., and Shapira, R., Nature, 204, 1273 (1965).

    Article  ADS  Google Scholar 

  23. Nakao, A., and Roboz-Einstein, E., Fed. Proc., 24, 247 (1965).

    Google Scholar 

  24. Roboz-Einstein, E., Robertson, D. M., DiCaprio, J. M., and Moore, W. J., J. Neurochem., 9, 353 (1962).

    Article  Google Scholar 

  25. Kibler, R. F., Ann. N.Y. Acad. Sci., 122, 194 (1965).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University of Leeds,

    P. R. CARNEGIE & C. E. LUMSDEN

  2. the Russell Grimwade School of Biochemistry, University of Melbourne, Victoria, Australia

    P. R. CARNEGIE & C. E. LUMSDEN

Authors
  1. P. R. CARNEGIE
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  2. C. E. LUMSDEN
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CARNEGIE, P., LUMSDEN, C. Encephalitogenic Peptides from Spinal Cord. Nature 209, 1354–1355 (1966). https://doi.org/10.1038/2091354a0

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