Abstract
REVERSIBLE and fixed L-forms of Agrobacterium tumefaciens have been obtained using glycine as inducing agent, and also with penicillin1,2. The characteristics of the bacterial strain play a definite part in the formation of spheroplasts. In A. tumefaciens this property is extremely important. The A.T. strain used by Rubio-Huertos and Beltrá readily formed spheroplasts which multiplied for years, even in media lacking glycine, without reverting to the normal bacterial form. By contrast, three other strains in the same conditions failed to do so.
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References
Rubio-Huertos, M., and Desjardins, P. R., Microbiol. Espan., 9, 375 (1956).
Rubio-Huertos, M., and Beltrá, R., Nature, 195, 101 (1962).
Challice, C. E., and Gorril, R. H., Biochim. Bioyhys. Acta, 14, 482 (1954).
Mandel, P., Sensenbrenner, M., and Vincendon, G., Nature, 182, 674 (1958).
Kelner, A. J., Bacteriology, 65, 252 (1953).
Barner, H. D., and Cohen, S. S., J. Bact., 71, 149 (1955).
Cooper, P. D., Bact. Revs., 20, 28 (1956).
Medill-Brown, M., and Hutchinson, W. C., J. Bact., 74, 280 (1957).
Scott, McN., and Chu, E., Exp. Cell. Res., 14, 166 (1958).
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RUBIO-HUERTOS, M., DE HERRERA, E. Fixed L-forms of Agrobacterium tumefaciens induced by Ultra-violet Light Radiation. Nature 209, 1262–1263 (1966). https://doi.org/10.1038/2091262b0
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DOI: https://doi.org/10.1038/2091262b0
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