Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Fixed L-forms of Agrobacterium tumefaciens induced by Ultra-violet Light Radiation

Abstract

REVERSIBLE and fixed L-forms of Agrobacterium tumefaciens have been obtained using glycine as inducing agent, and also with penicillin1,2. The characteristics of the bacterial strain play a definite part in the formation of spheroplasts. In A. tumefaciens this property is extremely important. The A.T. strain used by Rubio-Huertos and Beltrá readily formed spheroplasts which multiplied for years, even in media lacking glycine, without reverting to the normal bacterial form. By contrast, three other strains in the same conditions failed to do so.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Rubio-Huertos, M., and Desjardins, P. R., Microbiol. Espan., 9, 375 (1956).

    CAS  Google Scholar 

  2. Rubio-Huertos, M., and Beltrá, R., Nature, 195, 101 (1962).

    Article  ADS  CAS  Google Scholar 

  3. Challice, C. E., and Gorril, R. H., Biochim. Bioyhys. Acta, 14, 482 (1954).

    Article  CAS  Google Scholar 

  4. Mandel, P., Sensenbrenner, M., and Vincendon, G., Nature, 182, 674 (1958).

    Article  ADS  CAS  Google Scholar 

  5. Kelner, A. J., Bacteriology, 65, 252 (1953).

    CAS  Google Scholar 

  6. Barner, H. D., and Cohen, S. S., J. Bact., 71, 149 (1955).

    Google Scholar 

  7. Cooper, P. D., Bact. Revs., 20, 28 (1956).

    CAS  Google Scholar 

  8. Medill-Brown, M., and Hutchinson, W. C., J. Bact., 74, 280 (1957).

    CAS  PubMed  Google Scholar 

  9. Scott, McN., and Chu, E., Exp. Cell. Res., 14, 166 (1958).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

RUBIO-HUERTOS, M., DE HERRERA, E. Fixed L-forms of Agrobacterium tumefaciens induced by Ultra-violet Light Radiation. Nature 209, 1262–1263 (1966). https://doi.org/10.1038/2091262b0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/2091262b0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing