Abstract
IT has recently been reported, that lactate dehydrogenase isozymes differ from each other in denaturation caused by urea1–4, probably depending on the different resistance of the sub-units in these isozymes against denaturating forces. The aim of this contribution is to show that the presence of coenzyme during urea incubation may help to differentiate isozymes composed of different sub-units, and that the increase of substrate molarity will further accentuate this differentiation.
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LINDY, S., KONTTINEN, A. Reduced Nicotinamide-adenine Dinucleotide and Pyruvate in Urea-denaturation of Lactic Dehydrogenase Isozymes. Nature 209, 79–80 (1966). https://doi.org/10.1038/209079a0
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DOI: https://doi.org/10.1038/209079a0
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