Abstract
THE incorporation of a suitable quantity of tritiated water in the growth medium permits the continuous irradiation of cells cultured in vitro. A uniform source of β-radiation is thus provided which can be used in a variety of cell culture techniques. Some observations on the effects of continuous irradiation of HeLa cells grown as a monolayer at 37° C have already been published1,2. This communication reports some results of the continuous irradiation of HeLa cells at −196° C.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Nias, A. H. W., and Lajtha, L. G., Nature, 202, 613 (1964).
Nias, A. H. W., Gilbert, C. W., Lajtha, L. G., and Lange, C. S., Intern. J. Rad. Biol., 9, 275 (1965).
Nagington, J., and Greaves, R. I. N., Nature, 194, 993 (1962).
Puck, T. T., and Marcus, P. I., J. Exp. Med., 103, 653 (1956).
Stapleton, G. E., and Edington, C. W., Rad. Res., 5, 39 (1956).
Webb, R. B., Ehret, C. F., and Powers, E. L., Experientia, 14, 324 (1958).
Powers, E. L., Webb, R. B., and Ehret, C. F., Rad. Res., Supp. 2, 94 (1960).
Belli, J. A., and Bonte, F. J., Rad. Res., 18, 272 (1963).
Dawson, K. B., Field, E. O., and Madoc-Jones, H., Nature, 195, 1012 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
NIAS, A., LAJTHA, L. Continuous Irradiation of HeLa Cells at −196° C. Nature 208, 400–401 (1965). https://doi.org/10.1038/208400b0
Issue Date:
DOI: https://doi.org/10.1038/208400b0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
