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Acid Phosphatases

Nature volume 208, pages 382–383 (1965)Cite this article

Abstract

WE have been concerned with the isolation of acid phosphatases (orthophosphoric monoester phosphohydrolases, 3.1.3.2.) in quantities sufficient for chemical studies of these enzymes. The sources chosen were: the potato, the human prostate gland and human seminal plasma. We have found that the potato is a poor source and, although the enzyme has been obtained in a form which is homogeneous to starch-gel electrophoresis and to ultracentrifugation, the method is tedious and, because of the very large purification factor involved (about 25,000), leads to a low final yield (about 10 mg from 50 kg of potatoes). The best preparation previously reported was apparently associated with a purification factor of about 1,000 (ref. 1).

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References

  1. Andreu, M., Fernandez Alvarez, E., and Lora-Tamayo, M., Anales real soc. espan. fis y quim. (Madrid), 56B, 67 (1960).

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Authors and Affiliations

  1. Department of Chemistry,

    C. A. VERNON, J. GAULDIE, JENNIFER M. HANSON, J. M. HUMPHREYS & P. E. SMITH

  2. Department of Pharmacology,

    A. J. LAWRENCE

  3. Department of Physiology, University College, London, W.C.1.

    BARBARA E. C. BANKS

Authors
  1. C. A. VERNON
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  2. J. GAULDIE
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  3. JENNIFER M. HANSON
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  4. J. M. HUMPHREYS
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  5. P. E. SMITH
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  6. A. J. LAWRENCE
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  7. BARBARA E. C. BANKS
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VERNON, C., GAULDIE, J., HANSON, J. et al. Acid Phosphatases. Nature 208, 382–383 (1965). https://doi.org/10.1038/208382b0

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