Abstract
FREEZE-FIXATION implies rapid freezing of small specimens followed by vapour fixation at a low temperature. This method differs from freeze-drying by not removing the water of the specimen before fixation, and from the freeze-substitution by not replacing the water during fixation. All these three methods work with frozen tissue, but since the mode of fixation varies the ultrastructure of the cellular constituents also varies. Therefore, further information of the cellular constituents might be obtained.
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NILSSON, O. Freeze-fixation, a New Method for Electron Microscopy. Nature 208, 90 (1965). https://doi.org/10.1038/208090a0
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DOI: https://doi.org/10.1038/208090a0
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