Letter | Published:

Genetic Recombination with Ethyl-methanesulphonate-induced Waxy Mutants in-Maize

Naturevolume 207pages890891 (1965) | Download Citation



INTRACISTRON mapping in maize has been demonstrated by Nelson1,2 with a technique based on differential staining reactions of starch in pollen of different genotypes. Those with the waxy (wx) locus stain reddish brown in iodine–potassium iodide solutions; whereas non-waxy (Wx) pollen stains dark blue. Therefore, in waxy plants genetic recombination or back-mutation at this locus is manifested by the appearance of dark blue staining pollen. The technique has a distinct advantage in genetic investigations with higher plants in that large populations of hundreds of thousands of genotypes (pollen grains) can be scored with ease.

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  1. 1

    Nelson, Oliver E., Science, 130, 794 (1959).

  2. 2

    Nelson, Oliver E., Genetics, 47, 737 (1962).

  3. 3

    Amano, E., and Smith, H. H., Mutation Research (in the press).

  4. 4

    Briggs, R. W., and Smith, H. H., J. Heredity (in the press).

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  1. Department of Biology, Brookhaven National Laboratory, Upton, New York

    • R. W. BRIGGS
    • , E. AMANO
    •  & H. H. SMITH


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