Letter | Published:

Precipitation of Salmon Sperm Deoxyribonucleic Acid with Purine-specific Antibody

Naturevolume 207pages858860 (1965) | Download Citation

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Abstract

DEOXYRIBONUCLEIC acids (DNA) have been found to react with antisera prepared by immunizing animals to Gram-negative bacteria1, DNA2, nucleosides3–5, nucleotides4, purines5–7 and pyrimidines5,8. This was first demonstrated by complement fixation1,6 and then by precipitin reaction3–5. The ease and degree to which these reactions occur seem to depend on the source of the DNA. Pneumo-coccal and E. coli DNA, undenatured and denatured, fix complement with pyrimidine specific antibody; but these and calf thymus DNA are reported to require heat denaturation for complement fixation with anti-purinoyl antibody, although some complement fixation was observed with undenatured pneumococcal and E. coli DNA6,8. For precipitation, calf thymus DNA required heat denaturation to bring down anti-calf thymus2 or anti-uridine antibody3 and heat denaturation in the presence of formaldehyde to precipitate with anti-purinoyl, anti-pyrimidine or anti-nucleoside antibody5. In contrast, heat-denatured chick embryo DNA precipitated both with anti-purinoyl and anti-nucleoside antibody5. The difference in reactivity between calf thymus and chick embryo DNA was accounted for on the basis of the lack of hyperchromicity for heat-denatured chick embryo DNA and formaldehyde denatured calf thymus DNA, cycled slowly through the thermal transition temperature, compared to a hyperchromicity of 19.5 per cent for heat-denatured calf thymus DNA treated similarly5.

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Author notes

    • H. H. WEETALL

    Present address: Space General Corporation, El Monte, California

Affiliations

  1. Space Sciences Division, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California

    • H. H. WEETALL
    •  & N. WELIKY

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https://doi.org/10.1038/207858a0

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