Letter | Published:

Loss of Methyl Tritium from [3H] Acetate in Rumen Fluid

Naturevolume 207pages760761 (1965) | Download Citation



THE availability of both [14C]- and [3H]-labelled compounds, together with the development and refinement of scintillation counting procedures, has encouraged the use of radioisotopes in the investigation of metabolic processes. It has generally been assumed that with carbon-14 little or no isotope effect is produced and that the labelled substrate behaves in a similar way to the non-labelled compound. With tritium, however, considerable isotope effect may occur because of the large differences in atomic weights of tritium and hydrogen1.

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  1. 1

    Calvin, M., Heidelberger, C., Reid, J. C., Tolbert, B. M., and Yankwich, P. E., in Isotopic Carbon (Chapman and Hall, Ltd., London, 1949).

  2. 2

    Annison, E. F., in Metabolism and Physiological Significance of Lipids, edit. by Dawson, R. M. C., and Rhodes, D. N. (Wiley and Co., London, 1964).

  3. 3

    Davis, C. L., and Brown, R. E., J. Dairy Sci., 44, 1179 (1961).

  4. 4

    Hendler, R. W., Anal. Biochem., 7, 110 (1964).

  5. 5

    Annison, E. F., and Lewis, D., Metabolism in the Rumen (Methuen and Co., Ltd., London, 1959).

  6. 6

    Gray, F. V., Jones, G. B., and Pilgrim, A. F., Austral. J. Agric. Res., 11, 383 (1960).

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  1. Department of Biochemistry and Nutrition, University of New England, Armidale, New South Wales

    • R. A. LENG
    •  & G. J. LEONARD


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