Letter | Published:

Salicyluric Acid as a Metabolite of L-Tryptophan

Naturevolume 207page757 (1965) | Download Citation

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Abstract

THE metabolites appearing in the urine after an oral load of L-tryptophan have been separated by paper, thin-layer and column chromatography. After ingestion of L-tryptophan (70 mg/kg body-wt.) the urine was collected at timed intervals over the next 24 h. Aliquots of the urine were examined by two-way paper chromatography (n-butanol–acetic acid–water, 12 : 3 : 5, followed by 20 per cent KCl in water), and by thin-layer chromatography on silica gel using chloroform, methanol, acetic acid (76 : 22 : 2 by volume). The metabolites were located with Ehrlich and Pauly reagents1 and by fluorescence in ultra-violet light at two wave-lengths (253 and 366 mµ).

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References

  1. 1

    Smith, I., Chromatographic and Electrophoretic Techniques, 1, 193, 218, 298 (London: Heinemann, 1960).

  2. 2

    Komrower, G. M., Wilson, V., Clamp, J. R., and Westall, R. G., Arch. Dis. Childhood, 39, 250 (1964).

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Affiliations

  1. Department of Medicine, University of Bristol

    • W. I. AUSTAD
    •  & J. R. CLAMP
  2. Medical Unit, University College Hospital Medical School, London, W.C.1

    • R. G. WESTALL

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https://doi.org/10.1038/207757a0

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