Letter | Published:

Salicyluric Acid as a Metabolite of L-Tryptophan

Naturevolume 207page757 (1965) | Download Citation



THE metabolites appearing in the urine after an oral load of L-tryptophan have been separated by paper, thin-layer and column chromatography. After ingestion of L-tryptophan (70 mg/kg body-wt.) the urine was collected at timed intervals over the next 24 h. Aliquots of the urine were examined by two-way paper chromatography (n-butanol–acetic acid–water, 12 : 3 : 5, followed by 20 per cent KCl in water), and by thin-layer chromatography on silica gel using chloroform, methanol, acetic acid (76 : 22 : 2 by volume). The metabolites were located with Ehrlich and Pauly reagents1 and by fluorescence in ultra-violet light at two wave-lengths (253 and 366 mµ).

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Smith, I., Chromatographic and Electrophoretic Techniques, 1, 193, 218, 298 (London: Heinemann, 1960).

  2. 2

    Komrower, G. M., Wilson, V., Clamp, J. R., and Westall, R. G., Arch. Dis. Childhood, 39, 250 (1964).

Download references

Author information


  1. Department of Medicine, University of Bristol

    • W. I. AUSTAD
    •  & J. R. CLAMP
  2. Medical Unit, University College Hospital Medical School, London, W.C.1

    • R. G. WESTALL


  1. Search for W. I. AUSTAD in:

  2. Search for J. R. CLAMP in:

  3. Search for R. G. WESTALL in:

About this article

Publication history

Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.