Abstract
NEARLY all media routinely used for cell culture contain from 5 to 40 per cent serum of a particular species of animal. As one might expect, there has been much research into the nature of the fraction or fractions of serum which form the active part of this material1–6. Despite this the exact nature of the activity of serum or its fractions is unknown.
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References
Sanford, K. K., Westfall, B. B., Fioramonti, M. C., McQuilkin, W. T., Bryant, J. C., Peppers, F. V., Evans, V. J., and Earle, W. R., J. Nat. Cancer Inst., 16, 789 (1955).
Holmes, R., and Wolfe, S. W., J. Biophys. Biochem. Cytol., 10, 389 (1961).
Dupree, L. T., Sanford, K. K., Westfall, B. B., and Covalesky, A. B., Exp. Cell Res., 28, 381 (1962).
Fisher, H. W., Puck, T. T., and Sato, G., J. Exp. Med., 109, 649 (1959).
Lieberman, I., Lamy, F., and Ove, P., Science, 129, 43 (1959).
Tozer, B. T., and Pirt, J. S., Nature, 201, 375 (1964).
Holmes, R., J. Biophys. Biochem. Cytol., 6, 535 (1959).
Waymouth, C., J. Nat. Cancer Inst., 22, 1003 (1959).
Evans, V. J., Bryant, J. C., Fioramonti, M. C., McQuilkin, W. T., Sanford, K. K., and Earle, W. R., Cancer Res., 16, 77 (1956).
Evans, V. J., Bryant, J. C., McQuilkin, W. T., Fioramonti, M. C., Sanford, K. K., Westfall, B. B., and Earle, W. R., Cancer Res., 10, 87 (1956).
McQuilkin, W. T., Evans, V. J., and Earle, W. R., J. Nat. Cancer Inst., 19, 885 (1957).
Bakken, P. C., Evans, V. J., Earle, W. R., and Stevenson, R. E., Amer. J. Hyg., 73, 96 (1961).
Evans, V. J., LaRock, J. F., Vosida, T. H., and Potter, M., Exp. Cell Res., 32, 212 (1963).
Merchant, D. J., and Hellman, K. B., Proc. Soc. Exp. Biol. and Med., 110, 194 (1962).
Morgan, J. F., Morton, H. J., and Parker, R. C., Proc. Soc. Exp. Biol. and Med., 73, 1 (1950).
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PUMPER, R., YAMASHIROYA, H. & MOLANDER, L. Growth of Mammalian Cells in a Heat-stable Dialysable Medium. Nature 207, 662–663 (1965). https://doi.org/10.1038/207662a0
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DOI: https://doi.org/10.1038/207662a0
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