Letter | Published:

Demonstration of 5-Hydroxytryptamine in Blood Platelets by Electron Microscope Autoradiography

Naturevolume 207pages650651 (1965) | Download Citation



MAMMALIAN blood platelets are known to contain 5-hydroxytryptamine (5-HT) and to concentrate 5-HT added to platelet rich plasma in vitro1. Hughes and Brodie2 suggested that 5-HT existed in an unbound form in the platelet. The maintenance of increased platelet 5-HT was believed to be best explained by the presence of an active transport system for 5-HT2,3. The finding that the amount of 5-HT in platelets is related to the content of adenosine triphosphate (ATP) has led to the suggestion that the amine is bound to ATP4, and investigations using density-gradient centrifugation of disintegrated platelets have demonstrated a fraction containing most of the 5-HT and ATP5. More recently, in experiments with platelets disrupted ultrasonically and subjected to differential centrifugation 80 per cent of the 5-HT was recovered in the sedimented fraction, suggesting that 5-HT was bound within granules6.

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  1. 1

    Hardisty, R. M., and Stacey, R. S., J. Physiol., 130, 711 (1955).

  2. 2

    Hughes, F. B., and Brodie, B. B., J. Pharm. Exp. Therap., 127, 96 (1959).

  3. 3

    Weissbach, H., and Redfield, B. G., J. Biol. Chem., 235, 3287 (1960).

  4. 4

    Born, G. V. R., Ingram, G. I. C., and Stacey, R. S., Brit. J. Pharmacol., 13, 62 (1958).

  5. 5

    Baker, R. V., Blaschko, H., and Born, G. V. R., J. Physiol., 149, 55P (1959).

  6. 6

    Buckingham, S., and Maynert, E. W., J. Pharm. Exp. Therap., 143, 332 (1964).

  7. 7

    Salpeter, M. M., and Bachmann, L., J. Cell Biol., 22, 469 (1964).

  8. 8

    Aster, R. H., and Jandl, J. H., J. Clin. Invest., 43, 843 (1964).

  9. 9

    Fischman, D. A., and Gershon, M. D., J. Cell Biol., 21, 139 (1964).

  10. 10

    Koehler, J. K., Muhlethaler, K., and Freywyssling, A., J. Cell Biol., 16, 73 (1963).

  11. 11

    Chalkley, H. W., J. Nat. Cancer Inst., 4, 47 (1943).

  12. 12

    Holcenberg, J., and Benditt, E. P., Lab. Invest., 10, 144 (1961).

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  1. Sir William Dunn School of Pathology, University of Oxford

    • R. B. DAVIS
    •  & D. KAY


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