Abstract
MAMMALIAN blood platelets are known to contain 5-hydroxytryptamine (5-HT) and to concentrate 5-HT added to platelet rich plasma in vitro1. Hughes and Brodie2 suggested that 5-HT existed in an unbound form in the platelet. The maintenance of increased platelet 5-HT was believed to be best explained by the presence of an active transport system for 5-HT2,3. The finding that the amount of 5-HT in platelets is related to the content of adenosine triphosphate (ATP) has led to the suggestion that the amine is bound to ATP4, and investigations using density-gradient centrifugation of disintegrated platelets have demonstrated a fraction containing most of the 5-HT and ATP5. More recently, in experiments with platelets disrupted ultrasonically and subjected to differential centrifugation 80 per cent of the 5-HT was recovered in the sedimented fraction, suggesting that 5-HT was bound within granules6.
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References
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DAVIS, R., KAY, D. Demonstration of 5-Hydroxytryptamine in Blood Platelets by Electron Microscope Autoradiography. Nature 207, 650–651 (1965). https://doi.org/10.1038/207650a0
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DOI: https://doi.org/10.1038/207650a0
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