Letter | Published:

Demonstration of 5-Hydroxytryptamine in Blood Platelets by Electron Microscope Autoradiography

Naturevolume 207pages650651 (1965) | Download Citation

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Abstract

MAMMALIAN blood platelets are known to contain 5-hydroxytryptamine (5-HT) and to concentrate 5-HT added to platelet rich plasma in vitro1. Hughes and Brodie2 suggested that 5-HT existed in an unbound form in the platelet. The maintenance of increased platelet 5-HT was believed to be best explained by the presence of an active transport system for 5-HT2,3. The finding that the amount of 5-HT in platelets is related to the content of adenosine triphosphate (ATP) has led to the suggestion that the amine is bound to ATP4, and investigations using density-gradient centrifugation of disintegrated platelets have demonstrated a fraction containing most of the 5-HT and ATP5. More recently, in experiments with platelets disrupted ultrasonically and subjected to differential centrifugation 80 per cent of the 5-HT was recovered in the sedimented fraction, suggesting that 5-HT was bound within granules6.

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References

  1. 1

    Hardisty, R. M., and Stacey, R. S., J. Physiol., 130, 711 (1955).

  2. 2

    Hughes, F. B., and Brodie, B. B., J. Pharm. Exp. Therap., 127, 96 (1959).

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    Weissbach, H., and Redfield, B. G., J. Biol. Chem., 235, 3287 (1960).

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    Born, G. V. R., Ingram, G. I. C., and Stacey, R. S., Brit. J. Pharmacol., 13, 62 (1958).

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    Baker, R. V., Blaschko, H., and Born, G. V. R., J. Physiol., 149, 55P (1959).

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    Buckingham, S., and Maynert, E. W., J. Pharm. Exp. Therap., 143, 332 (1964).

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    Salpeter, M. M., and Bachmann, L., J. Cell Biol., 22, 469 (1964).

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    Aster, R. H., and Jandl, J. H., J. Clin. Invest., 43, 843 (1964).

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    Fischman, D. A., and Gershon, M. D., J. Cell Biol., 21, 139 (1964).

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    Koehler, J. K., Muhlethaler, K., and Freywyssling, A., J. Cell Biol., 16, 73 (1963).

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Affiliations

  1. Sir William Dunn School of Pathology, University of Oxford

    • R. B. DAVIS
    •  & D. KAY

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https://doi.org/10.1038/207650a0

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