Letter | Published:

Mechanism of Choline Sulphate Utilization in Fungi

Naturevolume 207pages632634 (1965) | Download Citation

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Abstract

CHOLINE sulphate is synthesized by higher fungi1, algae2 and plants3. In fungi it is found in high concentration in the mycelium4 and in spores5 and is thought to act as a store of sulphur6,7 and possibly carbon and nitrogen. Synthesis of choline sulphate6,8 involves activation of inorganic sulphate by the enzymes ATP-sulphate adenylyltransferase (EC.2.7.7.4), (ATP-sulphurylase)and ATP-adenylyl 3′-phosphotransferase (EC.2.7.1.25), (APS-kinase), to give adenosine 3′-phosphate 5′-sulphatophosphate (PAPS) and the subsequent transfer of the sulphate group of PAPS to choline catalysed by 3′-phosphoadenylylsulphate: choline sulphotransferase (EC. 2.8.2) (choline sulphotransferase)9. The mechanism of the utilization of the sulphur of choline sulphate for cysteine synthesis has been in some doubt. A reversal of the choline sulphotransferase reaction to give PAPS which could then be converted to SO32− and then to cysteine has been ruled out9,10. Several workers have failed to detect a choline sulphatase in fungi11,12, and while this enzyme has been detected in A. sydowi and P. chrysogenum13, the activity of the enzyme was very low and its physiological significance uncertain. Evidence is now presented confirming the presence of choline sulphatase in fungi and establishing that this enzyme is obligatory for choline sulphate utilization.

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Affiliations

  1. Department of Biochemistry, Trinity College, University of Dublin

    • CAROLINE HUSSEY
    • , BRUNO A. ORSI
    • , JOHN SCOTT
    •  & BRIAN SPENCER

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https://doi.org/10.1038/207632b0

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