An Inhibitor of Oxytocin from the Urinary Bladder of the Toad, Bufo marinus


A PREVIOUS investigation has shown that the isolated bladder of the toad, B. marinus, releases an inhibitor of oxytocin into the bathing Ringer's solution1. More recently a similar inhibitor has been invoked to explain the inhibition of the effects of vasopressin and of adenosine-3′, 5′-monophosphate on the toad bladder2. In the former investigation it was shown that inhibitor could be obtained by gently stirring isolated toad bladders in a modified Ringer's solution, and that the inhibitor so obtained was not readily dialysable. In the investigation reported here the previous results were confirmed; however, it was found that, following heat-treatment of the inhibitor solution, the inhibitor became dialysable. We report a modified preparation of the inhibitor based on this finding.

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  1. 1

    Karlin, A., Biochem. Biophys. Res. Comm., 11, 44 (1963).

  2. 2

    Edelman, I. S., Petersen, M. J., and Gulyassy, P. F., J. Clin. Invest., 43, 2185 (1964).

  3. 3

    Bentley, P. J., J. Endocrinol., 17, 201 (1958).

  4. 4

    Craig, L. C., Science, 144, 1093 (1964).

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KARLIN, A., OVERWEG, N. An Inhibitor of Oxytocin from the Urinary Bladder of the Toad, Bufo marinus. Nature 207, 1401–1402 (1965).

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