Bioassay of Pituitary Gonadotropins


DURING the course of purification of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from horse pituitary glands1 the following method was used to determine the degree of separation of FSH and LH at two purification steps. A pH-5 soluble gonadotropic fraction and a purified FSH preparation from the third zone-electrophoresis were compared as to their effect on the increase in the weight of ovaries as a measure of FSH and ventral prostates as a measure of LH in hypophysectomized rats (Holtzman Co., Madison, Wisconsin). A dose-response relationship was developed between the ovarian weights obtained by different doses of the pH-5 soluble fraction. The doses of the FSH fraction from the third zone-electrophoresis were selected so as to induce essentially the same ovarian response as was obtained with various doses of the pH-5 soluble fraction. The average weights of the ventral prostates produced by these doses were used as a measure of the LH content of the two fractions. The results are presented in Fig. 1 and Table 1.

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  1. 1

    Saxena, B. B., McShan, W. H., and Meyer, Roland K., Biochim. Biophys. Acta, 65, 394 (1962).

  2. 2

    Goldstein, A., Biostatistics, 144 (Macmillan Co., New York, 1964).

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SAXENA, B. Bioassay of Pituitary Gonadotropins. Nature 207, 1198–1199 (1965).

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