Abstract
THE plaque techniques of Jerne et al.1 and of Ingraham and Bussard2 for assaying single antibody-producing cells involve the lysis of erythrocytes suspended in three dimensions around the active cells in a supporting medium, agar or methyl cellulose. Maximum sensitivity with this system would be expected if a single layer of lymphoid cells and target erythrocytes was examined. A method has been devised which dispenses with supporting media and allows detection of cells producing antibody sufficient to lyse only 10–20 adjacent erythrocytes.
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References
Jerne, N. K., Nordin, A. A., and Henry, C., in Cell-bound Antibodies, edit. by Amos, B., and Koprowski, H., 109 (Wistar Inst. Press, Philadelphia, 1963).
Ingraham, J. S., and Bussard, A., J. Exp. Med., 119, 667 (1964).
Halliday, W. J., and Webb, M., Austral. J. Exp. Biol. Med. Sci., 43, 163 (1965).
Smith, J. B., and Cunningham, A. J. (to be published).
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CUNNINGHAM, A. A Method of Increased Sensitivity for detecting Single Antibody-forming Cells. Nature 207, 1106–1107 (1965). https://doi.org/10.1038/2071106a0
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DOI: https://doi.org/10.1038/2071106a0
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