A Method of Increased Sensitivity for detecting Single Antibody-forming Cells

Abstract

THE plaque techniques of Jerne et al.1 and of Ingraham and Bussard2 for assaying single antibody-producing cells involve the lysis of erythrocytes suspended in three dimensions around the active cells in a supporting medium, agar or methyl cellulose. Maximum sensitivity with this system would be expected if a single layer of lymphoid cells and target erythrocytes was examined. A method has been devised which dispenses with supporting media and allows detection of cells producing antibody sufficient to lyse only 10–20 adjacent erythrocytes.

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References

  1. 1

    Jerne, N. K., Nordin, A. A., and Henry, C., in Cell-bound Antibodies, edit. by Amos, B., and Koprowski, H., 109 (Wistar Inst. Press, Philadelphia, 1963).

  2. 2

    Ingraham, J. S., and Bussard, A., J. Exp. Med., 119, 667 (1964).

  3. 3

    Halliday, W. J., and Webb, M., Austral. J. Exp. Biol. Med. Sci., 43, 163 (1965).

  4. 4

    Smith, J. B., and Cunningham, A. J. (to be published).

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CUNNINGHAM, A. A Method of Increased Sensitivity for detecting Single Antibody-forming Cells. Nature 207, 1106–1107 (1965). https://doi.org/10.1038/2071106a0

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