Letter | Published:

Method for demonstrating the Chromosomes in Adult Human Spleen Cells

Naturevolume 207pages10081009 (1965) | Download Citation



To obtain chromosome preparations from in vitro cultures of human peripheral blood is now a routine laboratory procedure. Baker and Atkins1 and Bain and Gauld2 have extended this technique to human foetal and infant spleen and adult lymph nodes. The present communication describes the further extension of this method, to demonstrate the chromosomes of adult human spleen cells following culture in vitro.

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  1. 1

    Baker, M. C., and Atkins, N. B., Lancet, i, 1164 (1963).

  2. 2

    Bain, A. D., and Gauld, I. K., Brit. J. Exp. Path., 45, 530 (1964).

  3. 3

    Woodruff, M. F. A., The Transplantation of Tissues and Organs (Thomas, C. C., Springfield, Ill., 1960).

  4. 4

    Fox, M., and Zeiss, I. M., Nature, 192, 1213 (1961).

  5. 5

    Woodruff, M. F. A., and Nolan, B., Lancet, ii, 689 (1961).

  6. 6

    Woodruff, M. F. A., and Nolan, B., Lancet, ii, 426 (1963).

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  1. Department of Surgery, University of Bristol, Royal Infirmary, Bristol, 2

    • G. N. LUMB
    •  & M. O. SYMES


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