A Deoxyribonuclease from Mammary Tumours of C3H Mice preferentially hydrolysing Heat-denatured DNA

Abstract

ENZYMES hydrolysing heat-denatured DNA exclusively, or at a faster rate than native DNA, have been reported in the literature. Two are of bacterial origin1,2, two of mammalian origin3,4, and one from the venom of Viperalebotina5. Burdon, Smellie and Davidson identified one such enzyme, in extracts of rat liver, as being an inhibitor of the synthesis of DNA by hydrolysing the DNA primer in the DNA polymerase reaction4. If the function of these enzymes is to destroy DNA templates, their significance is obvious, even though it would be hard to assign such a role to the extracellular micrococcal nuclease1, or to the gurza venom enzyme5.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Cunningham, L., Catlin, B. W., and Privat de Garilhe, M., J. Amer. Chem. Soc., 78, 4642 (1956).

  2. 2

    Lehman, I. R., J. Biol. Chem., 235, 1479 (1960).

  3. 3

    Healy, I. W., Stollar, D., and Levine, L., in Methods in Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 6, 49 (Academic Press, New York and London, 1964).

  4. 4

    Burdon, M. G., Smellie, R. M. S., and Davidson, J. N., Biochim. Biophys. Acta, 91, 46 (1964).

  5. 5

    Nikolskaya, I. I., Shalina, N. M., and Tikhonenko, T. I., Biochim. Biophys. Acta, 91, 354 (1964).

  6. 6

    Kay, E. R. M., Simmons, N. S., and Dounce, A. L., J. Amer. Chem. Soc., 74, 1724 (1952).

  7. 7

    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

  8. 8

    Dische, Z., in The Nucleic Acids, edit. by Chargaff, E., and Davidson, J. N., 1, 285 (Academic Press, New York and London, 1955).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

GEORGATSOS, J., SYMEONIDIS, A. A Deoxyribonuclease from Mammary Tumours of C3H Mice preferentially hydrolysing Heat-denatured DNA. Nature 206, 1362–1363 (1965). https://doi.org/10.1038/2061362a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.