3′,5′-Ditritiomethotrexate as a Granulocyte Label

Abstract

THE folic acid antagonist methotrexate (amethopterin; 4-amino-4-deoxy-10-methylpteroylglutamate), when administered to human subjects, binds tightly to the enzyme dihydrofolate reduetase., present in immature leucocytes and in developing erythrocyte forms1–3. Bioassay of bound methotrexate has shown that the compound appears to remain with the leucocyte throughout its life-span1,2. In this communication we are reporting the synthesis of tritium-labelled methotrexate (MTX-3H) of very high specific activity (15.4 c./mmole), and its use as a tag in studies of granulocyte kinetics.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Bertino, J. R., Donohue, D. M., Simmons, B., Silber, R., Gabrio, B. W., and Huennekens, F. M., J. Clin. Invest., 42, 466 (1963).

  2. 2

    Bertino, J .R., Cancer Res., 23, 1286 (1963).

  3. 3

    Bertino, J. R., Hollingsworth, J. W., and Cashmore, A. R., Trans. Amer. Assoc. Physicians, 76, 63 (1963).

  4. 4

    Andrews, K. J. M., Bultitude, F., Evans, E. A., Gronow, M., Lambert, R. W., and Marrian, D. H., J. Chem. Soc., 3440 (1962).

  5. 5

    Johns, D. G., Sperti, S., and Burgen, A. S. V., J. Clin. Invest., 40, 1684 (1961).

  6. 6

    Oliverio, V. T., Anal. Chem., 33, 263 (1961).

  7. 7

    Johns, D. G., Hollingsworth, J. W., Cashmore, A. R., Plenderleith, I. H., and Bertino, J. R., J. Clin. Invest., 43, 621 (1964).

  8. 8

    Rabinowitz, Y., Blood, 23, 811 (1964).

  9. 9

    Reviewed by Cronkite, E. P., and Fliedner, T. M., New England J. Med., 270, 1347 (1964); 270, 1403 (1964).

  10. 10

    Samuels, J. D., Kisieleski, W. E., and Baserga, R., Atompraxis, 10, 144 (1964).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

BERTINO, J., JOHNS, D., ALMQUIST, P. et al. 3′,5′-Ditritiomethotrexate as a Granulocyte Label. Nature 206, 1052–1053 (1965). https://doi.org/10.1038/2061052b0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.