Abstract
THE kidney has been shown by several workers1–3 to produce an erythropoietic substance. Investigations of the renal site of erythropoietin production, however, have been controversial and inconclusive7–6. The known antigenic properties of erythropoietin7 prompted us to try a line of investigation using the fluorescent antibody technique. Anti-erythropoietin serum was prepared by injecting a young Dutch rabbit with concentrated sheep erythropoietin in complete Freund's adjuvant. (Step III Sheep Erythropoietin Lot No. K 147192 with specific activity of 11.7 units per mg protein was supplied by the U.S.P.H.S. Hematology Study Section.) 0.4 ml. of the antigen–adjuvant emulsion containing 4.8 mg (56 units) erythropoietin was injected intradermally into the footpads (0.1 ml. into each pad) of the rabbit on the first day of the investigation. One week later a second dose of 0.4 ml. (4.8 mg protein) was injected intradermally into 4 separate sites on the back of the neck. A control rabbit was also injected in the same way with Freund's adjuvant alone. Two weeks later the rabbits were bled and the sera separated.
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References
Jacobson, L. O., Goldwasser, E., Fried, W., and Plzak, L. F., Nature, 179, 633 (1957).
Fisher, J. W., and Birdwell, B. J., Acta Hæmat., 16, 224 (1961).
Kuratowska, Z., Lewartowski, B., Michalak, E., Blood, 18, 527 (1961).
Osnes, S., Brit. Med. J., 2, 1387 (1958).
Hirashima, K., and Takaku, F., Blood, 20, 2 (1962).
Muirhead, E. E., Fifth Ann. Sci. Meet. Ann. Hem. Soc. (Columbus, Ohio) (1962).
Lange, R. D., Gardner, jun., E., Wright, C. S., and Gallagher, N. J., Brit. J. Hæmat., 10, 69 (1964).
Ouchterlony, O., Progr. Allergy, 6, 1 (1958).
Jacobson, L. O., Plzak, L. F., and Fried, W., Proc. Soc. Exp. Biol. and Med., 94, 243 (1957).
Nairn, R. C., Chadwick, C. S., and McEntegart, M. G., J. Path. Bact., 76, 143 (1958).
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FISHER, J., TAYLOR, G. & PORTEOUS, D. Localization of Erythropoietin in Glomeruli of Sheep Kidney by Fluorescent Antibody Technique. Nature 205, 611–612 (1965). https://doi.org/10.1038/205611a0
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DOI: https://doi.org/10.1038/205611a0
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