Letter | Published:

Infra-nuclear Distribution of Transaminases

Nature volume 204, pages 880881 (28 November 1964) | Download Citation

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Abstract

IT has previously been shown1 that isolated calf thymus nuclei prepared by the sucrose-CaCl2 procedure2 contain a free amino-acid pool. Of the free amino-acids, glutamic acid represents about 40 per cent. The presence of such a relatively large amount of glutamic acid suggests its rapid synthesis, or its formation by metabolic conversion from other substances, such as those resulting from trans-aminase-catalysed reactions. The intracellular distribution of transaminase activity has been extensively investigated, particularly in rat liver3–6, and most of the enzymatic activity has been found in the mitochondrial fraction. Little is yet known, however, about the intranuclear distribution of transaminases. Techniques for separating nuclear proteins into various morphologically-defined fractions made possible the present investigation, undertaken to assay the calf thymus cells for two transaminase activities. It will be shown that the cell nucleus contains significant transaminase activities, mostly localized in the nuclear sap, resolvable by chromatography.

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Affiliations

  1. Department of Pharmacology, St. Louis University School of Medicine, St. Louis, Mo.

    • KUANG MEI WANG
  2. Department of Biology, State University of New York, Buffalo.

    • TUNG-YUE WANG

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https://doi.org/10.1038/204880a0

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