Letter | Published:

Tritium Labelling of Antibodies

Nature volume 204, pages 796797 (21 November 1964) | Download Citation

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Abstract

A LARGE number of alkylating agents react with proteins while forming relatively stable compounds. Ethyl methane sulphonate (EMS) reacts in proteins mainly with non-ionized amino groups and ionized sulphydryl- and carboxyl-groups1. Below pH 8, the carboxyl groups constitute the majority of the alkylated groups, and above this pH-level the amino groups are also alkylated. By varying the pH it is possible to vary the pattern of the groups which are alkylated2, and this can be utilized to attach tritium-labelled groups to proteins. In the work recorded here we used tritium-labelled EMS, which was synthesized from commercially available tritiated ethanol (ethanol-2-T, Radiochemical Centre, Amersham) and methanesulphonic anhydride.

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References

  1. 1.

    , and , Biochem. Pharmacol., 1, 25 (1958).

  2. 2.

    , Biological Alkylating Agents (Butterworths, London, 1962).

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Author information

Affiliations

  1. Institute of Radiobiology, Royal University of Stockholm.

    • C.-G. ROSÉN
    • , L. EHRENBERG
    •  & G. AHNSTRÖM

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DOI

https://doi.org/10.1038/204796a0

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