Abstract
TPNH generation in immature and adult rat testis in vivo is under hormonal control1,2. Greenberg and Glick, working with ACTH injections in the adrenal cortex, also found increased TPN reduction3. There is biochemical evidence that reduced TPN is required for steroid synthesis in vitro. Lynn and Brown4 showed that TPNH is essential for testosterone microsomic synthesis. Hall and Eik-Nes5 demonstrated in the rabbit testis in vitro that TPNH accelerates the conversion of 14C cholesterol to 14C testosterone. The experiment related in this communication was performed with the aim of elucidating whether or not reduced TPN is oxidized by the immature rat testis, and if this function is under hormonal control. Immature animals were selected because in them the dilution factors that interfere with enzymatic assays in adult animals2 are not significant.
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References
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SCHOR, N., PEREZ, A. TPNH Oxidation by the Prepuberal Rat Testis. Nature 204, 790–791 (1964). https://doi.org/10.1038/204790a0
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DOI: https://doi.org/10.1038/204790a0
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