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TPNH Oxidation by the Prepuberal Rat Testis

Naturevolume 204pages790791 (1964) | Download Citation



TPNH generation in immature and adult rat testis in vivo is under hormonal control1,2. Greenberg and Glick, working with ACTH injections in the adrenal cortex, also found increased TPN reduction3. There is biochemical evidence that reduced TPN is required for steroid synthesis in vitro. Lynn and Brown4 showed that TPNH is essential for testosterone microsomic synthesis. Hall and Eik-Nes5 demonstrated in the rabbit testis in vitro that TPNH accelerates the conversion of 14C cholesterol to 14C testosterone. The experiment related in this communication was performed with the aim of elucidating whether or not reduced TPN is oxidized by the immature rat testis, and if this function is under hormonal control. Immature animals were selected because in them the dilution factors that interfere with enzymatic assays in adult animals2 are not significant.

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  1. 1

    Schor, N. A., Cara, J., and Perez, A., Nature, 198, 1310 (1963).

  2. 2

    Schor, N. A., and Perez, A. (in preparation).

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    Greenberg, L. J., and Glick, D., J. Biol. Chem., 235, 3028 (1960).

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    Lynn, jun., S. W., and Brown, R. H., J. Biol. Chem., 232, 1015 (1958).

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    Ochoa, S., ibid., 1, 699.

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    Conover, T. E., Danielson, L., and Ernster, L., Biochim. Biophys. Acta, 67, 254 (1963).

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    Schor, N. A., Perez, A., and Schneider, N. O. (in preparation).

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    Hayano, M., and Dorfman, R. I., in Methods in Enzymology, 5, 503 (Academic Press, New York, 1962).

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    Phillips, A. H., and Langdon, R. G., J. Biol. Chem., 237, 2652 (1962).

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    • N. A. SCHOR

    Present address: Department of Pathology, Stanford University Medical School, Palo Alto, California


  1. Institute of Cellular Biology, Universidad Nacional de Cordoba, Argentina

    • N. A. SCHOR
    •  & A. PEREZ


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