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Detection of Enzymes in Agar Electropherograms

Nature volume 204, page 684 (14 November 1964) | Download Citation



To demonstrate the proteolytic enzymes in an electropherogram, Uriel et al.1,2 dipped an electropherogram into a substrate solution of an appropriate pH and, after an incubation period of some hours, the electropherogram was stained with amido black. Light spots indicated the presence of proteolytic enzymes. In their immunological work, Raunio and Gabriel3 used a second slide covered with substrate in agar to find the precipitation lines that had proteolytic activity. Here, too, the disappearance of substrate through enzymatic activity is demonstrated by staining the protein. With these publications in mind, we developed a simple and rapid method for localizing enzymatic activities in agar electropherograms.

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    , , and , Bull. Soc. Chim. Biol., 42, 1285 (1960).

  2. 2.

    , Nature, 188, 853 (1960).

  3. 3.

    , and , Nature, 197, 1012 (1963).

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    , Biochim. Biophys. Acta, 62, 445 (1962).

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  1. Laboratory of Histology and Microscopical Anatomy, State University, Utrecht.

    • C. POORT
    •  & W. J. W. VAN VENROOY


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