Letter | Published:

Use of Mutants to study the Electron Transport Pathway of Nitrate Reductase

Naturevolume 204pages575576 (1964) | Download Citation



NITRATE-INDUCIBLE TPNH (reduced triphosphopyridine nucleotide) cytochrome c reductase is believed to participate in nitrate reduction for the following reasons: (1) Sorger1 and Kinsky and McElroy2 showed that it is induced and repressed simultaneously with nitrate reductase by the same specific inducers and repressors. (2) Kinsky and McElroy2 observed that cytochrome c competes with nitrate in purified preparations of nitrate reductase. (3) These investigators also found that inhibitors of TPNH cytochrome c reductase inhibit nitrate reductase. (4) They further observed that the ratio of TPNH cytochrome c reductase to nitrate reductase remains constant throughout a fifty to seventy-fold purification by two procedures. (5) Sorger1,3 and Pateman et al.4 found that mutants at a single locus, lacking nitrate reductase, also lack nitrate-inducible TPNH cytochrome c reductase. (6) Sorger5 noted that the ratio of TPNH cytochrome c reductase to nitrate reductase remains constant during heat inactivation (at 31.1° C and 42.3° C) of crude extracts made from induced mycelium.

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  1. 1

    Sorger, G. J., Biochem. Biophys. Res. Comm., 12, 395 (1963).

  2. 2

    Kinsky, S., and McElroy, W. D., Arch. Biochem. Biophys., 73, 466 (1958).

  3. 3

    Sorger, G. J., Genetics, 48, 911 (1963).

  4. 4

    Pateman, J. A., Cove, D. J., Rever, B. M., and Roberts, D. B., Nature, 201, 58 (1964).

  5. 5

    Sorger, G. J., Ph.D. thesis, Yale University, 1964.

  6. 6

    Martin, R. G., and Ames, B. N., J. Biol. Chem., 236, 1372 (1961).

  7. 7

    Evans, H. J., and Nason, A., Arch. Biochem. and Biophys., 39, 234 (1952).

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  1. School of Science, Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon



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