Letter | Published:

Possible Cause of Electrophoretic and Chromatographic Heterogeneity of Pituitary Hormones

Nature volume 204, pages 574575 (07 November 1964) | Download Citation

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Abstract

THERE is evidence that pituitary hormones can exist in more than one biologically active form. Electrophoresis1 or chromatography2 of thyrotrophic hormone has been found to yield a number of active fractions. Several components with corticotrophic potency were obtained from adrenocorticotrophic hormone (ACTH) from various sources3–5, while fractionation of ovine prolactin also revealed various active forms of the hormone6,7. Similar results have been obtained with preparations of sheep8,9 and human10 interstitial cell-stimulating hormone (ICSH), as well as with alkali-treated growth hormone from beef pituitary glands11. A comparative study of the electrophoretic behaviour of pituitary hormones has been published by Ferguson and Wallace12, who reported that preparations of pituitary hormones were invariably electrophoretically impure and that more than seventy components could be detected in some crude extracts of pituitary tissue by electrophoresis on starch gel.

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Affiliations

  1. Department of Biochemistry, Antoni van Leeuwenhoek-Huis, The Netherlands Cancer Institute, Amsterdam.

    • M. SLUYSER

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https://doi.org/10.1038/204574b0

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