Abstract
THE observation that commercial blue-black ink poured on to the uncolonized agar of a Petri dish culture of Trichophyton mentagrophytes became discoloured in the region of the dermatophyte, but not of a contaminating mould, led to further investigation of this phenomenon. A solution of one of the major constituents of blue-black ink—ink blue (supplied by Imperial Chemical Industries as ‘Ink Blue AS’ crystals)—behaved similarly, and it seemed probable that the clearing was caused by either pH or Eh conditions around the colony. The response of Ink Blue to changes of this kind was initially examined in aqueous solution.
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References
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Cheaters, C. G. C., and Mathison, G. E., Sabouraudia, 2, 225 (1963).
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BAXTER, M., COOPER, J. Ink Blue as a pH Indicator for Certain Biological Systems. Nature 204, 1105–1106 (1964). https://doi.org/10.1038/2041105a0
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DOI: https://doi.org/10.1038/2041105a0
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