Abstract
SYNCHRONIZED cultures offer advantages in investigations of the sequence of biochemical changes occurring during the life-cycle of the mammalian cell. Such investigations suffer generally because present methods of producing synchronized cells do not provide immediately sufficient material to permit the use of classical analytical techniques. This communication describes a method by which gram quantities of synchronized cells representing a predetermined segment of the life-cycle can be produced.
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References
Xeros, N., Nature, 194, 682 (1962).
Puck, T. T., Science, 144, 565 (1964).
Bootsma, D., Budke, L., and Vos, O., Exp. Cell Res., 33, 301 (1964).
Tjio, J. H., and Puck, T. T., J. Exp. Med., 108, 259 (1958).
Puck, T. T., Sanders, P. C., and Petersen, D. F., Biophys. J. (in the press).
Engelberg, J., Exp. Cell Res., 23, 218 (1961).
Puck, T. T. (personal communication).
Puck, T. T., and Steffen, J., Biophys. J., 3, 379 (1963).
Anderson, E. C., and Petersen, D. F. (in preparation).
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PETERSEN, D., ANDERSON, E. Quantity Production of Synchronized Mammalian Cells in Suspension Culture. Nature 203, 642–643 (1964). https://doi.org/10.1038/203642a0
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DOI: https://doi.org/10.1038/203642a0
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