Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Local and Systemic Anaphylaxis in the Pertussis-inoculated Mouse


IT is now well known that mice injected with Bordetella pertussis vaccine plus an antigen will produce more antibodies to that antigen than mice injected with antigen alone1,2. Such mice also develop an enhanced susceptibility to anaphylactic shock1–3. However, the level of circulating antibodies in an animal as measured by agglutination, hæmagglutination, or precipitin techniques appears to be unrelated to the susceptibility of that animal to systemic anaphylaxis1,2,4. The following experiment indicates that the incidence of active cutaneous anaphylaxis in the pertussis-inoculated mouse parallels the incidence of systemic anaphylaxis. Mice were sensitized with 5 billion B. pertussis organisms and 1 mg of bovine serum albumin. On the seventh day after sensitization, 10 µg bovine serum albumin in 0.05 ml. of saline and 0.05 ml. of saline were injected into separate areas of the skin of the back. Fifteen min later, 0.25 ml. of 0.5 per cent Evans blue dye was injected into a lateral tail vein. Thirty min after the dye, the animals were challenged intraperitoneally with 2 mg of bovine serum albumin. Of 50 mice challenged in this manner, 32 died of anaphylaxis within 30 min. The survivors were classified as having symptoms of anaphylaxis or as having no symptoms and were killed 30 min after challenge. All animals were then tested for active cutaneous anaphylaxis by inspecting the inner side of the skin of the back for accumulation of dye at the site of antigen injection.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Get just this article for as long as you need it


Prices may be subject to local taxes which are calculated during checkout


  1. Kind, L. S., J. Immunol., 79, 238 (1957).

    CAS  PubMed  Google Scholar 

  2. Munoz, J., J. Immunol., 90, 132 (1963).

    CAS  PubMed  Google Scholar 

  3. Malkiel, S., and Hargis, B. J., Proc. Soc. Exp. Biol. and Med., 80, 122 (1952).

    Article  CAS  Google Scholar 

  4. Mota, I., Fed. Proc., 22, 559 (1963).

    Google Scholar 

  5. Mota, I., Immunol., 5, 11 (1962).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

KIND, L., RICHARDS, W. Local and Systemic Anaphylaxis in the Pertussis-inoculated Mouse. Nature 202, 309–310 (1964).

Download citation

  • Issue Date:

  • DOI:


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing