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Presence of Sulphated Nucleotides in the Epiphysial Growth Cartilage of the Rat


THE administration of radiosulphate to young rats is followed by a quick incorporation of sulphur-35 in the epiphysis of the long bones1–5. Chondroitin 35S-sulphate is isolated. The radioactivity of chondroitin sulphate reaches its maximum 24 h after the injection5–6. The enzymatic nature of this process has been firmly settled by Boström's experiments1,7,8. The work of Lipmann et al. has precisely shown the enzymatic mechanism which effects in vitro the synthesis of sulphated mucopolysaccharides by the formation of intermediate ‘active sulphate’ 3′-phosphoadenosine-5′-phosphosulphate (PAPS)9,10.

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PICARD, J., GARDAIS, A. & DUBERNARD, L. Presence of Sulphated Nucleotides in the Epiphysial Growth Cartilage of the Rat. Nature 202, 1213–1214 (1964).

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