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Inhibitory Effect of ‘Diamox’ and Chloroquine on the NADH2-Monodehydroascorbic Acid-transhydrogenase Activity of Retinal and Ciliary Microsomes


CYANIDE-INSENSITIVE, ascorbic acid-dependent NADH2 oxidation has recently been demonstrated in plant1,2 and animal3 tissues. It has been postulated3 that ascorbic acid is oxidized by cytochrome b5 to monodehydroascorbic acid, which is later reduced to ascorbic acid by NADH2-monodehydroascorbic acid-transhydrogenase. As the oxygen uptake of the retina4, ciliary body5, lens6 and cornea7 is not completely inhibited by cyanide and these tissues all contain high levels of ascorbic acid8, it was decided to investigate the occurrence of this enzyme in ocular tissues.

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HEATH, H., FIDDICK, R. Inhibitory Effect of ‘Diamox’ and Chloroquine on the NADH2-Monodehydroascorbic Acid-transhydrogenase Activity of Retinal and Ciliary Microsomes. Nature 202, 1128–1129 (1964).

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