Abstract
DESPITE the considerable variety of plant tissues already cultured in vitrothere are few instances of the identification of specific secondary metabolites in such cultures. Although Nicotiana1 and Atropa2 alkaloids have been found in the root organ cultures used in biosynthetic investigations, estimates2,3 of the concentration of these alkaloids in callus tissue were made without rigorous identification.
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References
Dawson, R. F., Amer. J. Bot., 29, 813 (1942). Solt, M. L., Dawson, R. F., and Christman, D. R., Plant Physiol., 35, 887 (1960). Schröter, H. B., and Engelbrecht, L., Arch. Pharm., 290, 204 (1957).
West, F. R., and Mika, E. S., Bot. Gaz., 119, 50 (1957). van Haga, P. R., Pharm. Weekblad., 89, 71 (1954). van Haga, P. R., Abhandl. deut. Akad. Wiss., Berlin, Kl. Chem., Geol. u. Biol., No. 7, 102 (1956).
Dawson, R. F., Amer. Scientist, 48, 336 (1960).
White, P. R., The Cultivation of Animal and Plant Cells (Ronald Press Co., New York, 1954).
Willits, C. O., Swain, M. L., Connelly, J. A., and Brice, B. A., Anal. Chem., 22, 430 (1950).
Porter, W. L., Naghski, J., and Eisner, A., Arch. Biochem., 24, 461 (1949).
Tso, T. C., and Jeffrey, R. N., Arch. Biochem. Biophys., 43, 269 (1953).
Tewari, S. N., Naturwiss., 41, 217 (1954).
Jeffery, R. N., and Eoff, W. H., Anal. Chem., 27, 1903 (1955).
Kuffner, F., Schick, K., and Bühn, H., Monatsh., 87, 749 (1956).
Murashige, T., and Skoog, F., Physiol. Plant., 15, 473 (1962).
Gänshirt, H., in Dünnschicht-Chromatographie, edit. by Stahl, E., 336 (Springer, 1962).
Dawson, R. F., Amer. J. Bot., 29, 66, 813 (1942).
Solt, M. L., Plant Physiol., 32, 484 (1957).
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SPEAKE, T., MCCLOSKEY, P., SMITH, W. et al. Isolation of Nicotine from Cell Cultures of Nicotian tabacum. Nature 201, 614–615 (1964). https://doi.org/10.1038/201614a0
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DOI: https://doi.org/10.1038/201614a0
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