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Polysaccharide Fractions and Transformations in Rhizobium

Naturevolume 199page927 (1963) | Download Citation



OUR interest in the composition of rhizobial gums1,2 led of to attempt transformations using, first a preparation based on that of Ljunggren3 and later the cruder, and therefore deoxyribonucleic acid-rich, fraction used by Lange and Alexander4. In the first case we aimed at restoring virulence to avirulent strains of clover Rhizobia, including two used by Ljunggren, but with R. trifolii, SU297/31 as the source of ‘polysaccharide’. In the second we attempted a wider transformation, similar to those reported by Lange and Alexander, between clover and lucerne. In the latter we included a specially prepared deoxyribonucleic acid fraction as an additional treatment.

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  1. 1

    Humphrey, Beverley A., and Vincent, J. M., J. Gen. Microbiol., 21, 477 (1959).

  2. 2

    Humphrey, Beverley A., Nature, 184, 1802 (1959).

  3. 3

    Ljunggren, H., Nature, 191, 623 (1961).

  4. 4

    Lange, R. T., and Alexander, M., Canad. J. Microbiol., 7, 959 (1961).

  5. 5

    Ephrussi-Taylor, H., Adv. Virus Res., 3, 275 (1955).

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  1. Microbiology Laboratory, School of Agriculture, University of Sydney

    •  & J. M. VINCENT


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