Letter | Published:

A Barley Endosperm Bioassay for Gibberellins

Naturevolume 199pages823824 (1963) | Download Citation



SINCE the review of bioassay techniques for gibberellins by Phinney and West1, several new techniques have been proposed2–4. These are based on the increased elongation of plant tissues induced by gibberellins. Recent work has defined other biological characteristics of gibberellic acid (GA3), notably a stimulation of the hydrolytic enzymes in germinating cereal grains5. Paleg6–8 demonstrated that this response resides in the cereal endosperm, and the bioassay reported here was developed from procedures used in those studies.

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  1. 1

    Phinney, B. O., and West, C. A., Ann. Rev. Plant Physiol., 11, 411 (1960).

  2. 2

    Frankland, B., and Wareing, P. F., Nature, 185, 255 (1960).

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    Wheeler, A. W., J. Exp. Bot., 11, 217 (1960).

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    Naylor, J. M., and Simpson, G. M., Nature, 192, 679 (1961).

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    Dahlstrom, R. V., and Sfat, M. R., Adv. Chem., Ser. 28, 59 (1960).

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    Paleg, L. G., Plant Physiol., 35, 293 (1960).

  7. 7

    Paleg, L. G., Plant Physiol., 35, 902 (1960).

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    Paleg, L. G., Plant Physiol., 36, 829 (1961).

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    Harada, H., and Nitsch, J. P., Plant Physiol., 34, 409 (1959).

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    Boothby, D., and Wright, S. T. C., Nature, 196, 389 (1962).

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    Present address: Division of Biology, California Institute of Technology, Pasadena


  1. Department of Plant Physiology, Waite Agricultural Research Institute, Adelaide

    •  & LESLIE G. PALEG


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