Letter | Published:

Reaction of Hydrogen Chloride Gas on RNA and its Component Nucleotides

Naturevolume 199pages811812 (1963) | Download Citation



THE release of purines and characteristic sequences of pyrimidine oligonucleotides from DNA has been achieved by the use of diphenylamine and formic acid1. In RNA the phosphodiester bonds are extremely acid and alkali-sensitive due to the ease with which cyclic phosphate intermediates are formed involving the 2′ hydroxyl group. In order to limit the possibility of cyclization an attempt was made to carry out a depurination reaction with RNA in the solid phase.

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  1. 1

    Burton, K., and Petersen, G. B., Biochem. J., 75, 17 (1960).

  2. 2

    Bramwell, M. E., and Jones, A. S., Nature, 195, 1095 (1962).

  3. 3

    Crestfield, A. M., Smith, K. C., and Allen, F. W., J. Biol. Chem., 216, 185 (1955).

  4. 4

    Levene, P. A., and Stiller, E. T., J. Biol. Chem., 104, 299 (1934).

  5. 5

    Hershey, A. D., Dixon, J., and Chase, M., J. Gen. Physiol., 36, 777 (1953).

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  1. Department of Cell Biology, John Innes Institute, Bayfordbury, Hertford

    • M. E. BRAMWELL


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