Letter | Published:

Preparation of Bovine Chromosomes

Naturevolume 199page719 (1963) | Download Citation

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Abstract

CERTAIN problems have induced us to prepare the chromosomes of bovine leucocytes. Using the methods of other authors1–3 the following technique was adopted: 20.0–40.0 ml. blood were collected from the jugular vein of an ox, to which was added 2.0–4.0 ml. heparin solution (200 mg heparin, 100 mg streptomycin, 10,000 units penicillin, 100.0 ml. of a 0.85 per cent saline solution) or an appropriate amount of ‘Vetren’ or ‘Thrombovetren’ (Promonta, Hamburg). This was put in a vessel containing 0.12–0.24 ml. phytohæmagglutinin P or 0.4–0.8 ml. phytohæmagglutinin M (Difco), well mixed, stored for 20 min in a refrigerator, centrifuged afterwards for 10 min at about 900g and the leucocyte-layer obtained. (Bovine erythrocytes were not agglutinated by phytohæmagglutinin.)

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References

  1. 1

    Edwards, J. H., and Young, R. B., Lancet, ii, 49 (1961).

  2. 2

    Kosenow, W., and Pfeiffer, R. A., Blut, 7, 500 (1961).

  3. 3

    Moorhead, P. S., Nowell, P. C., Mellman, W. J., Battips, D. M., and Hungerford, D. A., Exp. Cell Res., 20, 613 (1960).

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Affiliations

  1. Bundesgesundheitsamt, Abteilung für Veterinärmedizin, Berlin-Dahlem

    • F. ULBRICH
    •  & E. WEINHOLD
  2. Universitäts-Kinderklinik, Münster/Westfalen

    • R. A. PFEIFFER

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https://doi.org/10.1038/199719a0

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