Letter | Published:

Species Identification of Animal Cell Strains by Immunofluorescence

Naturevolume 199pages616617 (1963) | Download Citation

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Abstract

ANIMAL cells cultivated in vitro can be readily identified with respect to their species of origin by several immunologic procedures, including: hæmagglutination1, and mixed cell agglutination2, both involving detection of species antigens common to erythrocytes and cultured cells, and an immunofluorescence method3, which provides direct visualization of species-specific antigen–antibody reactions on cell surfaces. By means of these procedures, a number of established cell lines have been shown to be of a species other than that originally claimed4,5, a finding obviously due to heterologous cell contamination and overgrowth or other laboratory errors.

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References

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Affiliations

  1. Child Research Center of Michigan, Detroit, 2, Michigan

    • W. F. SIMPSON
    •  & C. S. STULBERG

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https://doi.org/10.1038/199616b0

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