Letter | Published:

Effect of Puromycin on Cholinesterase Activity of Embryonic Chick Intestine in Organ Culture

Naturevolume 199pages598599 (1963) | Download Citation



THE acetylcholinesterase (AChE) activity of a number of chick embryo tissues cultured in vitro can be influenced by the presence of acetylcholine (ACh) or other substrates in the nutrient media1–4. For example, in the presence of 0.02 M ACh, the AChE activity of primary explants of chick embryo lung increased 3–7-fold over a period of four days3. It would seem that a case of enzyme induction had been demonstrated. However, no direct evidence has been provided which would support this hypothesis. This report presents evidence that certain changes in AChE and cholinesterase (ChE) activity of chick embryo intestine maintained in vitro are due to changes in protein synthesis, a finding that is compatible with the hypothesis that the synthesis of AChE can be induced.

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    Present address: Department of Biochemistry, University of Glasgow,


  1. Department of Pharmacology, University of California, San Francisco Medical Center, San Francisco, 22, California



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