Abstract
THE acetylcholinesterase (AChE) activity of a number of chick embryo tissues cultured in vitro can be influenced by the presence of acetylcholine (ACh) or other substrates in the nutrient media1–4. For example, in the presence of 0.02 M ACh, the AChE activity of primary explants of chick embryo lung increased 3–7-fold over a period of four days3. It would seem that a case of enzyme induction had been demonstrated. However, no direct evidence has been provided which would support this hypothesis. This report presents evidence that certain changes in AChE and cholinesterase (ChE) activity of chick embryo intestine maintained in vitro are due to changes in protein synthesis, a finding that is compatible with the hypothesis that the synthesis of AChE can be induced.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jones, M., Featherstone, R. M., and Bonting, S. L., J. Pharmacol., 116, 114 (1956).
Burkhalter, A., Featherstone, R. M., Schueler, F. W., and Jones, M., J. Pharmacol., 120, 285 (1957).
Burkhalter, A., Jones, M., and Featherstone, R. M., Proc. Soc. Exp. Biol. Med., 96, 747 (1957).
Goodwin, B. C., and Sizer, I. W., Fed. Proc., 22 (2), 383 (1963).
Burkhalter, A. (in preparation).
Rabinowitz, M., and Fisher, J. M., J. Biol. Chem., 237, 477 (1962).
Takeda, Y., Hayashi, S.-I., Nakagawa, H., and Suzuki, F., J. Biochem. (Tokyo), 48, 169 (1960).
Ellman, G. L., Courtney, K. D., Andres, jun., V., and Featherstone, R. M., Biochem. Pharmacol., 7, 88 (1961).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BURKHALTER, A. Effect of Puromycin on Cholinesterase Activity of Embryonic Chick Intestine in Organ Culture. Nature 199, 598–599 (1963). https://doi.org/10.1038/199598a0
Issue Date:
DOI: https://doi.org/10.1038/199598a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
