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Incorporation of 5-Iodo-2′-deoxyuridine into the Deoxyribonucleic Acid of Vaccinia Virus

Nature 199, 1310–1311 (1963) | Download Citation

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Abstract

THE in vitro development of animal viruses containing deoxyribonucleic acid, such as vaccinia or herpes simplex, is inhibited by the thymidine analogue 5-iodo-2′-deoxyuridine (IUdR), whereas RNA-containing viruses are not affected1. This reagent has also been used successfully for the in vivo treatment of herpes2 and vaccinia3, and on tumours induced in hamsters by human adenovirus4. The mechanism of viral inhibition by IUdR is under investigation in these laboratories. Previous investigations5 suggest that one possible mechanism may be that replacement of thymidine by IUdR in viral DNA renders the virus non-infective. It has been shown that IUdR has no direct inactivating effect on vaccinia virus particles in vitro (unpublished results). This communication presents evidence for the incorporation of IUdR into the DNA of vaccinia virus.

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    Author information

    1. Y. S. BAKHLE: Fellow of the James Hudson Brown Fund.

    Affiliations

    1. Department of Pharmacology

      • WILLIAM H. PRUSOFF
      •  & Y. S. BAKHLE

    2. Department of Molecular Biology and Biophysics, Yale University, New Haven, Conn.

      • J. F. MCCREA

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    https://doi.org/10.1038/1991310a0

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