Letter | Published:

Elution Procedure for the Demonstration of Methæmoglobin in Red Cells of Human Blood Smears

Nature volume 199, pages 11961197 (21 September 1963) | Download Citation

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Abstract

THE peroxidatic activity of the red blood pigment can be inhibited by cyanide in the case of methæmoglobin (HbIII), but not in the case of oxyhæmoglobin (HbII) (ref. 1). It seemed promising to use this difference for a differential staining procedure of HbII- and HbIII-containing red cells by first adding cyanide to the heparinized or citrated blood in question and by staining smears prepared from it by a peroxidase reaction.

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References

  1. 1.

    , Biochem. Z., 321, 271 (1951).

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  1. Universitäts-Kinderklinik, Tübingen, Germany.

    • ENNO KLEIHAUER
    •  & KLAUS BETKE

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DOI

https://doi.org/10.1038/1991196b0

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