Letter | Published:

Reduction and Reoxidation of the Disulphide Bonds of Pepsinogen

Nature volume 199, pages 11861187 (21 September 1963) | Download Citation

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Abstract

Anfinsen and Haber have shown that the disulphide bonds of ribonuclease (RNase) can be cleaved by treatment of the native protein in 8 M urea with mercaptoethanol1. On exposure to molecular oxygen, reduced RNase is re-oxidized with complete regeneration of secondary and tertiary structure. The kinetics of the reaction indicate either that pairing of the correct half-cystine residues is a random process with the molecule undergoing subsequent structural rearrangement to yield the native form2; or, alternatively, that the reduced protein may be sufficiently structurally similar to the native enzyme to allow correct matching and reoxidation of the half-cystinyl residues, even in the absence of the disulphide bridges3.

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References

  1. 1.

    , and , J. Biol. Chem., 236, 1361 (1961).

  2. 2.

    , , , and , Proc. U.S. Nat. Acad. Sci., 47, 1309 (1961).

  3. 3.

    , and , J. Biol. Chem., 237, 1839 (1962).

  4. 4.

    , and , J. Biol. Chem., 238, 653 (1963).

  5. 5.

    , J. Amer. Chem. Soc., 76, 4331 (1954).

  6. 6.

    , in Crystalline Enzymes, edit. by Northrop, J. H., Kunitz, M., and Herriott, R. M., second ed., 305 (Columbia Univ. Press, New York, 1948).

  7. 7.

    , and , Chem Rev., 62, 457 (1962).

  8. 8.

    , J. Biol. Chem. (in the press).

  9. 9.

    , J. Gen. Physiol., 41, 441 (1958).

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Affiliations

  1. Department of Biochemistry, Georgetown University, Washington 7, D.C.

    • V. FRATTALI
  2. Physical Biochemistry Division, Naval Medical Research Institute, Bethesda 14, Md.

    • R. F. STEINER
    •  & D. B. S. MILLAR
  3. National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda 14, Md.

    • H. EDELHOCH

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https://doi.org/10.1038/1991186a0

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