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Resolution of a Racemic Pteridine during Paper Chromatography

Naturevolume 199pages10981099 (1963) | Download Citation



WHEN ethyl pyruvate and 4,5-diaminopyrimidine were refluxed in water, between pH 1 and 3, a solid of molecular weight about 320 was formed which was seen as two spots1 after dissolution in 0.1 N sodium hydroxide and chromatography on a sheet of Whatman No. 1 paper in water at pH 5.5 or 8.5. The spots (Fig. 1A) had RF values of 0.55 and 0.65 respectively and appeared identical when viewed in ultra-violet light. When eluted, and rechromatographed, each spot generated only one spot, which corresponded in RF to the spot from which it was eluted (Fig. 1B and C).

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  1. 1

    Albert, A., and Reich, F., J. Chem. Soc., 127 (1961).

  2. 2

    de Ligny, C., Nieboer, H., de Vijlder, J., and van Willigen, J., Rec. Trav. Chim. Pays-Bas, 82, 213 (1963). Rhuland, L., Work, E., Denman, R., and Hoare, D., J. Amer. Chem. Soc., 77, 4844 (1955). Blaschko, H., and Hope, D., Biochem. J., 63, 7P (1956). Weichert, B., Acta Chem. Scand., 9, 547 (1955). Closs, K., and Haug, C., Chem. and Indust., 103 (1953). Dalgliesh, C. E., J. Chem. Soc., 3940 (1952). Kotaki, M., Sakan, T., Nakamura, N., and Senoh, S., J. Amer. Chem. Soc., 73, 2973 (1951). Lambooy, J., ibid., 76, 133 (1954).

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  1. Department of Medical Chemistry, Australian National University, Canberra

    •  & E. P. SERJEANT


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