Letter | Published:

Resolution of a Racemic Pteridine during Paper Chromatography

Nature volume 199, pages 10981099 (14 September 1963) | Download Citation

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Abstract

WHEN ethyl pyruvate and 4,5-diaminopyrimidine were refluxed in water, between pH 1 and 3, a solid of molecular weight about 320 was formed which was seen as two spots1 after dissolution in 0.1 N sodium hydroxide and chromatography on a sheet of Whatman No. 1 paper in water at pH 5.5 or 8.5. The spots (Fig. 1A) had RF values of 0.55 and 0.65 respectively and appeared identical when viewed in ultra-violet light. When eluted, and rechromatographed, each spot generated only one spot, which corresponded in RF to the spot from which it was eluted (Fig. 1B and C).

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References

  1. 1.

    , and , J. Chem. Soc., 127 (1961).

  2. 2.

    , , , and , Rec. Trav. Chim. Pays-Bas, 82, 213 (1963). , , , and , J. Amer. Chem. Soc., 77, 4844 (1955). , and , Biochem. J., 63, 7P (1956). , Acta Chem. Scand., 9, 547 (1955). , and , Chem. and Indust., 103 (1953). , J. Chem. Soc., 3940 (1952). , , , and , J. Amer. Chem. Soc., 73, 2973 (1951). , ibid., 76, 133 (1954).

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Affiliations

  1. Department of Medical Chemistry, Australian National University, Canberra.

    • ADRIEN ALBERT
    •  & E. P. SERJEANT

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https://doi.org/10.1038/1991098a0

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