Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Immunofluorescent Test for Infectious Mononucleosis


THE laboratory diagnosis of infectious mononucleosis is based on the demonstration in the patient's serum of atypical mononuclear cells often accompanied by a rise in titre of heterophile agglutinins for sheep red blood cells1,2. It has been shown by Stuart et al.3 that these agglutinins may be differentiated from Forssman antibody and from naturally occurring sheep cell agglutinins by agglutination-inhibition tests, the antibody associated with glandular fever being absorbed by treated ox cells but not by an emulsion of guinea pig kidney.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. Paul, J. R., and Bunnell, W. W., Amer. J. Med. Sci., 183, 90 (1932).

    Article  Google Scholar 

  2. Hobson, F. G., Lawson, Barbara, and Wigfield, Mary, Brit. Med. J., i, 845 (1958).

  3. Stuart, C. A., Welch, H., Cunningham, J., and Burgess, A. M., Arch. Int. Med., 58, 512 (1936).

    Article  Google Scholar 

  4. Weir, D. M., Holborow, E. J., and Johnson, G. D., Brit. Med. J., i, 933 (1961).

  5. Grubb, R., and Swahn, B., Acta Path. Microbiol. Scand., 43, 305 (1958).

    Article  CAS  Google Scholar 

  6. Cohen, S., Nature, 197, 253 (1963).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and Permissions

About this article

Cite this article

JOHNSON, G., HOLBOROW, E. Immunofluorescent Test for Infectious Mononucleosis. Nature 198, 1316–1317 (1963).

Download citation

  • Issue Date:

  • DOI:

This article is cited by


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing