Abstract
THE proteinase of Bacillus larvae contains two components separable by paper chromatography in ascending phosphate buffer flow1,2. Preparative separation of the components was achieved with differential adsorption of the complex on cellulose powder and activated alumina. The chromatographiecally mobile component (I) was isolated following adsorption of the immobile component (II) on cellulose. Complete removal of II required several successive treatments with cellulose. The proteinase-containing extract could not be clarified by fritted glass filters without alteration of the properties of II.
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Minnesota Mining and Manufacturing Co., St. Paul, Minnesota.
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GOCHNAUER, T., YAMAMOTO, H. Separation of a Bacterial Proteinase on Fritted Glass Filters. Nature 198, 1226–1227 (1963). https://doi.org/10.1038/1981226a0
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DOI: https://doi.org/10.1038/1981226a0
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